| Titel |
Molekulare Physiologie der synaptischen Übertragung II  
|
| Title |
Molecular physiology of synaptic transmission II |
| Schwerpunkt/Focus |
|
| Sprache/Language |
englisch
deutsch |
| VV-Nr./Course No. |
136095 |
| Modulverantwortlich/Responsible |
J. Klingauf |
| Vertreter/Co-responsible |
|
| Anbieter/Teachers |
J. Klingauf; M. Kahms |
| Typ/Type |
Praktikum + Seminar |
| SWS/Semerster periods per week |
|
| Arbeitslast(h)/Work load |
300 h |
| KP/Credit points |
10 KP |
| Zuordnung/Classification |
Forschungs-Modul |
| Semester/Semester |
WiSe, SoSe |
| Studierende/Students |
MSc Biowissenschaften
MSc Biotechnologie
MSc Molekulare Biomedizin |
| Corona-Informationen/Corona-Information |
|
| Zeit/Date |
nach Vereinbarung |
| Ort/Location |
Institut für Medizinische Physik und Biophysik |
| Beginn/Start |
nach Vereinbarung |
| Vorbesprechung/Obligatory pre-meeting |
nach Vereinbarung |
| Voraussetzung/Prerequisite |
keine |
| Anmeldung/Registration |
über Modulverantwortlichen |
| Leistungskontrollen/Performance assessments |
Protokoll und Seminarvortrag |
| Termine f. Leistungskontrollen/Date for performance assessments |
|
| max. NP/Max. grade points |
200 NP |
| Ziele/Aims |
The object of this module is to introduce students to independent scientific work in preparation for a potential master thesis. Students will work on their own small project under supervision of an experienced scientist and learn modern live cell and super-resolution imaging techniques as well as cell physiological experimental strategies. The focus of research in our lab lies on synaptic vesicle recycling and projects will be related to the field of synaptic vesicle exo-endocytosis. |
| Inhalte/Content |
During synaptic transmission small synaptic vesicles filled with neurotransmitter fuse with the plasma membrane to release their content. For maintaining synaptic transmission the exocytosed vesicle proteins have to be retrieved thereafter by compensatory endocytosis. Though intensively studied, many aspects of the exo-endocytic itinerary are still vividly debated. What is the fate of synaptic vesicle proteins post fusion? How are exo- and compensatory endocytosis coupled in time and space? Are there different vesicle populations supporting different modes of synaptic activity?
During this module, the participants will gain knowledge about the basic molecular mechanisms involved synaptic vesicle recycling and learn about experimental strategies to analyze these mechanisms in more detail. These strategies include e.g. application of different pH-sensitive fluorophores specific for SV proteins to monitor the dynamics during stimulation-dependent exo-endocytosis, and high-resolution fluorescence microscopy using photo-switchable dyes to analyze localization of distinct synaptic proteins with nanometer precision.
|
| Methoden/Methods |
- preparation and cultivation of cells (cell lines, primary cells)
- molecular biology (PCR, cloning)
- transfection techniques
- live cell imaging (epi-fluorescence, total internal reflection microscopy)
- high resolution fluorescence microscopy (STORM microscopy, 4Pi microscopy) |
| Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills |
|
| Voraussetzung für/Prerequisite for |
|
| Präsenzpflicht/Compulsory presence |
ja/yes |
| Plätze/Number of participants |
2 |
| Gruppengröße/Group size |
|
| Materialien/Materials |
|
| Literatur/Literature |
- "Principals of neural science” Kandell, Schwartz and Jessel, 5th Edition, McGraw-Hill, 2012
- Wienisch M, Klingauf J. „Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical.” Nat Neurosci. 2006, 9(8):1019-27.
- Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J.“ A readily retrievable pool of synaptic vesicles.“ Nat Neurosci. 2011, 14(7):833-9.
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| Links |
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| Sonstiges/Further information |
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= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
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