Titel | Molekulare Physiologie der synaptischen Übertragung I |
Title | Molecular physiology of synaptic transmission |
Schwerpunkt/Focus | |
Sprache/Language | englisch deutsch |
VV-Nr./Course No. | 134032 |
Modulverantwortlich/Responsible | J. Klingauf |
Vertreter/Co-responsible | |
Anbieter/Teachers | J. Klingauf, M. Kahms |
Typ/Type | Praktikum + Seminar |
SWS/Semerster periods per week | |
Arbeitslast(h)/Work load | 150 h |
KP/Credit points | 5 KP |
Zuordnung/Classification | Fortgeschrittenen-Modul |
Semester/Semester | WiSe, SoSe |
Studierende/Students | MSc Biowissenschaften MSc Biotechnologie MSc Molekulare Biomedizin |
Corona-Informationen/Corona-Information | |
Zeit/Date | n.V. |
Ort/Location | Institut f. Medizinische Physik u Biophysik, Robert-Koch-Str. 31 |
Beginn/Start | |
Vorbesprechung/Obligatory pre-meeting | nach Vereinbarung |
Voraussetzung/Prerequisite | keine |
Anmeldung/Registration | über Modul-Verantwortliche/n |
Leistungskontrollen/Performance assessments | Protokoll und Seminarvortrag |
Termine f. Leistungskontrollen/Date for performance assessments | |
max. NP/Max. grade points | 200 NP |
Ziele/Aims | The focus of research in our lab lies on presynaptic synaptic vesicle recycling. This course is designed to teach the basic concepts and introduce the experimental tools of cellular neurophysiology through a combination of lecture time and hands-on-experience with neurophysiological techniques. |
Inhalte/Content | During synaptic transmission small synaptic vesicles filled with neurotransmitter fuse with the plasma membrane to release their content. For maintaining synaptic transmission the exocytosed vesicle proteins have to be retrieved thereafter by compensatory endocytosis. Though intensively studied, many aspects of the exo-endocytic itinerary are still vividly debated. What is the fate of synaptic vesicle proteins post fusion? How are exo- and compensatory endocytosis coupled in time and space? Are there different vesicle populations supporting different modes of synaptic activity? During this module, the participants will gain knowledge about the basic molecular mechanisms involved in synaptic vesicle recycling and learn about experimental strategies to analyze these mechanisms in more detail. These strategies include live cell imaging, high resolution fluorescence microscopy, patch clamp and electron microscopy. |
Methoden/Methods | - preparation and cultivation of cells (cell lines, primary cells, transfection techniques) - molecular biology (PCR, cloning) - live cell imaging (visualization of presynaptic exo-endocytosis) - high resolution fluorescence microscopy (STORM microscopy, STED microscopy) - patch clamp technique - electron microscopy |
Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills | |
Voraussetzung für/Prerequisite for | |
Präsenzpflicht/Compulsory presence | ja/yes |
Plätze/Number of participants | 2 |
Gruppengröße/Group size | |
Materialien/Materials | |
Literatur/Literature | “Synapses” Cowan, Südhof and Stevens, John Hopkins University Press, 1993 “Principals of neural science” Kandell, Schwartz and Jessel, 5th Edition, McGraw-Hill, 2012 |
Links | http://impb.uni-muenster.de/ |
Sonstiges/Further information |
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