Veranstaltung

Titel

Molekulare Physiologie der synaptischen Übertragung I

Title Molecular physiology of synaptic transmission
Schwerpunkt/Focus
Sprache/Language englisch deutsch
VV-Nr./Course No. 134032
Modulverantwortlich/Responsible J. Klingauf
Vertreter/Co-responsible
Anbieter/Teachers J. Klingauf, M. Kahms
Typ/Type Praktikum + Seminar
SWS/Semerster periods per week
Arbeitslast(h)/Work load 150 h
KP/Credit points 5 KP
Zuordnung/Classification Fortgeschrittenen-Modul
Semester/Semester WiSe, SoSe
Studierende/Students MSc Biowissenschaften
MSc Biotechnologie
MSc Molekulare Biomedizin
Corona-Informationen/Corona-Information
Zeit/Date n.V.
Ort/Location Institut f. Medizinische Physik u Biophysik, Robert-Koch-Str. 31
Beginn/Start
Vorbesprechung/Obligatory pre-meeting nach Vereinbarung
Voraussetzung/Prerequisite keine
Anmeldung/Registration über Modul-Verantwortliche/n
Leistungskontrollen/Performance assessments Protokoll und Seminarvortrag
Termine f. Leistungskontrollen/Date for performance assessments
max. NP/Max. grade points 200 NP
Ziele/Aims The focus of research in our lab lies on presynaptic synaptic vesicle recycling. This course is designed to teach the basic concepts and introduce the experimental tools of cellular neurophysiology through a combination of lecture time and hands-on-experience with neurophysiological techniques.
Inhalte/Content During synaptic transmission small synaptic vesicles filled with neurotransmitter fuse with the plasma membrane to release their content. For maintaining synaptic transmission the exocytosed vesicle proteins have to be retrieved thereafter by compensatory endocytosis. Though intensively studied, many aspects of the exo-endocytic itinerary are still vividly debated. What is the fate of synaptic vesicle proteins post fusion? How are exo- and compensatory endocytosis coupled in time and space? Are there different vesicle populations supporting different modes of synaptic activity?
During this module, the participants will gain knowledge about the basic molecular mechanisms involved in synaptic vesicle recycling and learn about experimental strategies to analyze these mechanisms in more detail. These strategies include live cell imaging, high resolution fluorescence microscopy, patch clamp and electron microscopy.
Methoden/Methods - preparation and cultivation of cells (cell lines, primary cells, transfection techniques)
- molecular biology (PCR, cloning)
- live cell imaging (visualization of presynaptic exo-endocytosis)
- high resolution fluorescence microscopy (STORM microscopy, STED microscopy)
- patch clamp technique
- electron microscopy
Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills
Voraussetzung für/Prerequisite for
Präsenzpflicht/Compulsory presence ja/yes
Plätze/Number of participants 2
Gruppengröße/Group size
Materialien/Materials
Literatur/Literature “Synapses” Cowan, Südhof and Stevens, John Hopkins University Press, 1993

“Principals of neural science” Kandell, Schwartz and Jessel, 5th Edition, McGraw-Hill, 2012
Links http://impb.uni-muenster.de/
Sonstiges/Further information

Modulelemente:

Elemente of the module:
Titel/Title Zeit (von...bis)/Time (from...to) Ort(Raum)/Location
Übungen/Practical exercises
Vorlesung/Lecture
Seminare/Semeinars
Exkursionen/Excursions
Legende: / Legend:

= Modul gehört zum SPP Imoplant / Module is part of the SSP Imoplant
= Modul gehört zum SPP Evolution /Module is part of the SSP Evolution
= Modul gehört zum SPP Bioanalytics and Biochemistry /Module is part of the SSP Bioanalytics and Biochemistry
= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
= Modul gehört zum SPP Quantitative Cell Biology /Module is part of the SSP Quantitative Cell Biology