Titel | Molecular Protein Evolution |
Title | Molecular Protein Evolution |
Schwerpunkt/Focus | |
Sprache/Language | englisch |
VV-Nr./Course No. | 132253 |
Modulverantwortlich/Responsible | Dr. Andreas Lange |
Vertreter/Co-responsible | Prof. Dr. Erich Bornberg-Bauer |
Anbieter/Teachers | Prof. Dr. Erich Bornberg-Bauer, Dr. Andreas Lange, Dr. Bharat Ravi |
Typ/Type | Praktikum + Seminar |
SWS/Semerster periods per week | |
Arbeitslast(h)/Work load | 150 h |
KP/Credit points | 5 KP |
Zuordnung/Classification | Fortgeschrittenen-Modul |
Semester/Semester | SoSe |
Studierende/Students | MSc Biowissenschaften MSc Biotechnologie MSc Molekulare Biomedizin |
Corona-Informationen/Corona-Information | |
Zeit/Date | Block II: 15.05. - 16.06.2023 |
Ort/Location | SP4-lab 10 (Schlossplatz 4) Institute for Evolution and Biodiversity (IEB, Hüfferstraße 1) |
Beginn/Start | |
Vorbesprechung/Obligatory pre-meeting | |
Voraussetzung/Prerequisite | |
Anmeldung/Registration | online |
Leistungskontrollen/Performance assessments | Protokolle + Mitarbeit + Report + Präsentation |
Termine f. Leistungskontrollen/Date for performance assessments | |
max. NP/Max. grade points | 200 NP |
Ziele/Aims | Theory and practicals of various techniques used in molecular protein evolution like directed evolution, and generating libraries of protein variants, and enzyme engineering. Example proteins (beta lactamase [known], and de novo proteins [unknown]) will be used to design strategies for protein evolution. |
Inhalte/Content | This course is an introduction into the principles of molecular protein evolution, and experimental techniques to study protein evolution along with their applications in biotechnology. Laboratory techniques covered in the course focus on mutating proteins at specific sites (rational design) or at random sites (directed evolution), and selecting the mutated proteins for specific functions. |
Methoden/Methods | Laboratory techniques include site-saturation mutagenesis, error-prone PCR, site-directed mutagenesis, DNA shuffling, molecular cloning, bacterial growth assays, DNA purification, and DNA (Sanger) sequencing. Computational techniques will cover DNA sequence analysis and protein structure predictions using AlphaFold. |
Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills | |
Voraussetzung für/Prerequisite for | |
Präsenzpflicht/Compulsory presence | ja/yes |
Plätze/Number of participants | 8 |
Gruppengröße/Group size | Nach Vereinbarung |
Materialien/Materials | |
Literatur/Literature | Die Literatur für dieses Modul wird in der Vorbesprechung bzw. zu Modulbeginn individuell mit den Studierenden abgesprochen. |
Links | https://bornberglab.org/msc-teaching/, http://www.uni-muenster.de/Evolution/teaching/ssp.shtml |
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