| Titel |
Molecular Mechanisms controlling Germ Cell Development in Zebrafish 
|
| Title |
Molecular Mechanisms controlling Germ Cell Development in Zebrafish |
| Schwerpunkt/Focus |
|
| Sprache/Language |
englisch
deutsch |
| VV-Nr./Course No. |
134007 |
| Modulverantwortlich/Responsible |
Prof. Dr. Erez Raz |
| Vertreter/Co-responsible |
Ggf. Vertreter/in für Modulverantwortliche/n |
| Anbieter/Teachers |
Prof. Dr. Erez Raz/Dr. Felix Gunawan |
| Typ/Type |
Praktikum |
| SWS/Semerster periods per week |
|
| Arbeitslast(h)/Work load |
300 h |
| KP/Credit points |
10 KP |
| Zuordnung/Classification |
Forschungs-Modul |
| Semester/Semester |
WiSe, SoSe |
| Studierende/Students |
MSc Biowissenschaften
MSc Biotechnologie
MSc Molekulare Biomedizin |
| Corona-Informationen/Corona-Information |
Studierende müssen sich an unseren Hygieneplan halten |
| Zeit/Date |
8 Wochen |
| Ort/Location |
ZMBE, Institut für Zellbiologie, Von-Esmarch-Str. 56 |
| Beginn/Start |
n. A. |
| Vorbesprechung/Obligatory pre-meeting |
n. A. |
| Voraussetzung/Prerequisite |
'keine' |
| Anmeldung/Registration |
per e-mail: glaesner@uni-muenster.de |
| Leistungskontrollen/Performance assessments |
Protokoll |
| Termine f. Leistungskontrollen/Date for performance assessments |
nach Modulbeendigung |
| max. NP/Max. grade points |
200 NP |
| Ziele/Aims |
The students will gain experience in molecular biology, zebrafish embryological technics and in documenting results on microscopes. |
| Inhalte/Content |
The biological context of the work is cell fate specification and migration using zebrafish germ cells as an accessible in vivo model. The module includes experiments analyzing the effects of specific manipulations of embryos on cell migration and development. The manipulations include over-expression and knock down of specific genes by antisense oligonucleotides and RNA injections respectively. |
| Methoden/Methods |
The evaluation of the phenotype involves various techniques including standard fluorescence as well as confocal microscopy for monitoring cell behavior in the context of the live embryo (generation of time-lapse movies). |
| Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills |
|
| Voraussetzung für/Prerequisite for |
|
| Präsenzpflicht/Compulsory presence |
ja/yes |
| Plätze/Number of participants |
2 |
| Gruppengröße/Group size |
optional |
| Materialien/Materials |
|
| Literatur/Literature |
Raz, E. (2003)
Primordial germ-cell development: the zebrafish perspective.
Nature Reviews Genetics 4: 690-700.
Tarbashevich K., Raz E. (2010)
The nuts and bolts of germ-cell migration.
Current Opinion in Cell Biology, 22: 1–7.
|
| Links |
|
| Sonstiges/Further information |
|
= Modul gehört zum SPP Imoplant / Module is part of the SSP Imoplant
= Modul gehört zum SPP Evolution /Module is part of the SSP Evolution
= Modul gehört zum SPP Bioanalytics and Biochemistry /Module is part of the SSP Bioanalytics and Biochemistry
= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
= Modul gehört zum SPP Quantitative Cell Biology /Module is part of the SSP Quantitative Cell Biology