Titel | Embryonic stem cell and mouse developmental biology techniques |
Title | Embryonic stem cell and mouse developmental biology techniques |
Schwerpunkt/Focus | |
Sprache/Language | englisch |
VV-Nr./Course No. | 134346 |
Modulverantwortlich/Responsible | Dr. ivan Bedzhov |
Vertreter/Co-responsible | |
Anbieter/Teachers | Dr. ivan Bedzhov |
Typ/Type | Praktikum |
SWS/Semerster periods per week | |
Arbeitslast(h)/Work load | 300 h |
KP/Credit points | 10 KP |
Zuordnung/Classification | Forschungs-Modul |
Semester/Semester | WiSe, SoSe |
Studierende/Students | Msc Biowissenschaften Msc Biotechnologie MSc Molekulare Biomedizin |
Corona-Informationen/Corona-Information | |
Zeit/Date | |
Ort/Location | Max Planck Institute for Molecular Biomedicine |
Beginn/Start | n.A. |
Vorbesprechung/Obligatory pre-meeting | |
Voraussetzung/Prerequisite | |
Anmeldung/Registration | ivan.bedzhov@mpi-muenster.mpg.de |
Leistungskontrollen/Performance assessments | Präsentation |
Termine f. Leistungskontrollen/Date for performance assessments | |
max. NP/Max. grade points | 200 |
Ziele/Aims | The research module “Embryonic stem cell and mouse developmental biology techniques” provides hands-on experience with ES cell culture, genetic modifications of ES cells (e.g. CRSPR/Cas9, LoxP/Cre and Tet-On/Off systems), 3D culture models including artificial embryo-like structures, microscopy techniques, embryo micromanipulations techniques (microinjections, live imaging, generation of chimeras, de novo establishment of stem cell lines, etc), next generation sequencing methods and others. |
Inhalte/Content | Developmental and stem cell biology |
Methoden/Methods | genetic modifications of ES cells (e.g. CRSPR/Cas9, LoxP/Cre and Tet-On/Off systems), 3D culture models including artificial embryo-like structures, microscopy techniques, embryo micromanipulations techniques (microinjections, live imaging, generation of chimeras, de novo establishment of stem cell lines, etc), next generation sequencing methods and others |
Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills | |
Voraussetzung für/Prerequisite for | |
Präsenzpflicht/Compulsory presence | ja |
Plätze/Number of participants | 1 |
Gruppengröße/Group size | |
Materialien/Materials | |
Literatur/Literature | Die Literatur für dieses Modul wird in der Vorbesprechung bzw. zu Modulbeginn individuell mit den Studierenden abgesprochen. |
Links | https://www.mpi-muenster.mpg.de/176627/projects |
Sonstiges/Further information |
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