Veranstaltung

Titel

Molekulare Physiologie der synaptischen Übertragung II

Title Molecular physiology of synaptic transmission II
Schwerpunkt/Focus
Sprache/Language englisch deutsch
VV-Nr./Course No. 130070
Modulverantwortlich/Responsible J. Klingauf
Vertreter/Co-responsible
Anbieter/Teachers J. Klingauf; M. Kahms
Typ/Type Praktikum + Seminar
SWS/Semerster periods per week
Arbeitslast(h)/Work load 300 h
KP/Credit points 10 KP
Zuordnung/Classification Forschungs-Modul
Semester/Semester WiSe, SoSe
Studierende/Students MSc Biowissenschaften
MSc Biotechnologie
MSc Molekulare Biomedizin
Corona-Informationen/Corona-Information
Zeit/Date nach Vereinbarung
Ort/Location Institut für Medizinische Physik und Biophysik
Beginn/Start nach Vereinbarung
Vorbesprechung/Obligatory pre-meeting nach Vereinbarung
Voraussetzung/Prerequisite keine
Anmeldung/Registration über Modulverantwortlichen
Leistungskontrollen/Performance assessments Protokoll und Seminarvortrag
Termine f. Leistungskontrollen/Date for performance assessments
max. NP/Max. grade points 200 NP
Ziele/Aims The object of this module is to introduce students to independent scientific work in preparation for a potential master thesis. Students will work on their own small project under supervision of an experienced scientist and learn modern live cell and super-resolution imaging techniques as well as cell physiological experimental strategies. The focus of research in our lab lies on synaptic vesicle recycling and projects will be related to the field of synaptic vesicle exo-endocytosis.
Inhalte/Content During synaptic transmission small synaptic vesicles filled with neurotransmitter fuse with the plasma membrane to release their content. For maintaining synaptic transmission the exocytosed vesicle proteins have to be retrieved thereafter by compensatory endocytosis. Though intensively studied, many aspects of the exo-endocytic itinerary are still vividly debated. What is the fate of synaptic vesicle proteins post fusion? How are exo- and compensatory endocytosis coupled in time and space? Are there different vesicle populations supporting different modes of synaptic activity?
During this module, the participants will gain knowledge about the basic molecular mechanisms involved synaptic vesicle recycling and learn about experimental strategies to analyze these mechanisms in more detail. These strategies include e.g. application of different pH-sensitive fluorophores specific for SV proteins to monitor the dynamics during stimulation-dependent exo-endocytosis, and high-resolution fluorescence microscopy using photo-switchable dyes to analyze localization of distinct synaptic proteins with nanometer precision.
Methoden/Methods - preparation and cultivation of cells (cell lines, primary cells)
- molecular biology (PCR, cloning)
- transfection techniques
- live cell imaging (epi-fluorescence, total internal reflection microscopy)
- high resolution fluorescence microscopy (STORM microscopy, 4Pi microscopy)
Berufsrelevante und interdisziplinäre Komponenten/Occupational and interdisciplinary skills
Voraussetzung für/Prerequisite for
Präsenzpflicht/Compulsory presence ja/yes
Plätze/Number of participants 2
Gruppengröße/Group size
Materialien/Materials
Literatur/Literature - "Principals of neural science” Kandell, Schwartz and Jessel, 5th Edition, McGraw-Hill, 2012
- Wienisch M, Klingauf J. „Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical.” Nat Neurosci. 2006, 9(8):1019-27.
- Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J.“ A readily retrievable pool of synaptic vesicles.“ Nat Neurosci. 2011, 14(7):833-9.

Links
Sonstiges/Further information

Modulelemente:

Elemente of the module:
Titel/Title Zeit (von...bis)/Time (from...to) Ort(Raum)/Location
Übungen/Practical exercises
Vorlesung/Lecture
Seminare/Semeinars
Exkursionen/Excursions
Legende: / Legend:

= Modul gehört zum SPP Imoplant / Module is part of the SSP Imoplant
= Modul gehört zum SPP Evolution /Module is part of the SSP Evolution
= Modul gehört zum SPP Bioanalytics /Module is part of the SSP Bioanalytics
= Modul gehört zum SPP Neuroscience and Behaviour /Module is part of the SSP Neuroscience and Behaviour
= Modul gehört zum SPP Quantitative Cell Biology /Module is part of the SSP Quantitative Cell Biology